FastQCFastQC Report
Fri 29 Jul 2022
EGAF00006419495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006419495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123610215
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17723301.4338054504637825No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1799200.1455543136139679TruSeq Adapter, Index 5 (100% over 50bp)
CGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1661620.1344241655109167No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)