FastQCFastQC Report
Wed 8 Feb 2023
EGAF00006420441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006420441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73347
Sequences flagged as poor quality0
Sequence length101
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT35884.891815616180621TruSeq Adapter, Index 19 (97% over 38bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG9161.2488581673415409No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA4030.5494430583391277No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG1320.17996646079594258TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTAGCTCGTAT1010.13770161015447122TruSeq Adapter, Index 19 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph