FastQCFastQC Report
Wed 8 Feb 2023
EGAF00006420442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006420442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73347
Sequences flagged as poor quality0
Sequence length101
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT32244.395544466713021Illumina Single End PCR Primer 1 (97% over 34bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA8441.1506946432710268No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG3840.523538795042742No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC1380.18814675446848542Illumina Single End PCR Primer 1 (96% over 33bp)
ACACACACACACACACACACACACACACACACACACACACACACACACAC800.10907058230057126No Hit
GCCGCAACGCGTGGGGCAACCTGTCCTACGCCGACCTCATCACCAAGGCC800.10907058230057126No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)