FastQCFastQC Report
Thu 6 Oct 2022
EGAF00006436299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006436299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92438914
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA10015371.0834582067894047No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT3951590.4274812228971015No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT3672070.39724287544096415No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC3572190.3864379021155528No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA3416830.36963112742756804No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC3370710.3646418866409443No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT3366400.36417563278599313No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG2650820.2867645113182528No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT2292540.24800594260551354No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA2048880.22164691376620888No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC2042030.2209058838575278No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC2040990.2207933771268667No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG1949610.21090792996551214No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA1912120.2068522786842779No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA1795310.1942158255991627No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC1721590.18624082926807212No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC1683350.18210404332530344No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG1682030.18196124632100286No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG1680110.18175354158747473No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG1676220.18133272314298285No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG1663300.17993504337361643No Hit
CCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGAC1579730.17089447848770703No Hit
CCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG1514220.16380763625154662No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC1492400.16144715849863836No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCT1472510.15929546727474536No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG1460690.15801678500896277No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC1459600.15789886930086608No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA1430640.1547659895701501No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC1379920.14927912285944855No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA1369770.14818110044001598No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1357630.14686780071864539No Hit
CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTC1354480.1465270351402008No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG1349500.1459883009876122No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT1328870.14375655689767192No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG1328280.14369273096393148No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT1323620.1431886142669309No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA1314100.14215874496318726No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG1297520.14036512804553286No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG1296050.14020610410892537No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC1289450.13949211908742243No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC1285150.13902694702795837No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1280520.13852607571741918No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTT1239940.13413614963066312No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATA1231850.1332609770815784No Hit
GCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTAT1223020.13230575166644645No Hit
CTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCA1217330.13169021003427195No Hit
GTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT1179000.12754368793211915No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT1167760.12632774980458988No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA1155820.12503608599296182No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC1153920.1248305448504079No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT1151610.12458065009288187No Hit
GGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCAC1147540.12414035932962171No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC1133010.1225685104868281No Hit
TCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG1124370.12163383918595148No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT1124210.12161653045815747No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC1123320.12152025065980329No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT1114690.12058666115441381No Hit
GTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAA1112530.12035299332919468No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC1110080.12008795343484888No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA1066080.11532805329149583No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG1064680.11517660192329825No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC1056510.11429277501031655No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC1052380.11384599347413363No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACC1036820.11216271969616606No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA1025580.11094678156863677No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACC1023520.11072393169828888No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCT1006940.10893031478063449No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC1006880.10892382400771172No Hit
GTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTA1003980.10861010331644529No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG991720.10728382204922918No Hit
GCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATC991260.1072340594568214No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC981310.10615767294713133No Hit
TCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCC974750.10544801510757688No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC972970.10525545551086851No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG941860.10188998975042049No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT937380.10140534537218816No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC929450.10054748155089749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)