FastQCFastQC Report
Thu 6 Oct 2022
EGAF00006436305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006436305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61293831
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA18910323.0851913955255954No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC5291120.8632385859516596No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT4344900.7088641595921782No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT3964240.6467600303854396No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC3904720.6370494283511174No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG3467080.5656490944414945No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA3179350.5187063605144864No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG3085180.5033426610257075No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC3056030.4985868806275137No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC3053570.49818553518053066No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG3014340.4917852173410404No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC2955770.4822296064346182No Hit
GTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT2923490.476963171057133No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG2636570.43015258746022905No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG2568170.41899322625143137No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC2542620.4148247806537007No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG2514270.4101995191000543No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA2496650.4073248415488991No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT2483770.4052234881516869No Hit
GGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCAC2422670.39525511139938374No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA2377400.38786937628356105No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC2189950.3572871795205622No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT2079720.33930331422749543No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATA1773510.28934559499144374No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA1679160.2739525287626417No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACC1634630.26668752357802533No Hit
GTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTA1632900.2664052765766917No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC1566610.25559015882038766No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG1538100.25093879349783177No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC1526680.24907563699191848No Hit
CCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGA1484950.2422674477632178No Hit
CGCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACG1484100.24212877149088624No Hit
GTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAA1458410.23793748509535975No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC1447080.23608901195945803No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT1445340.2358051334725676No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT1436580.2343759521247742No Hit
GCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTAT1434380.234017025302269No Hit
CTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCA1418530.23143112069467478No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA1418510.23142785772356111No Hit
GGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCG1379570.2250748529652193No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG1337600.21822750808315441No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCC1317520.21495148508501616No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA1293410.21101797340747067No Hit
CCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT1293340.21100655300857274No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT1291140.21064762618606758No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC1283110.20933754328392365No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC1274400.20791651936391448No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG1263810.20618877615921904No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1254370.20464865379356043No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCG1236370.20171197979124522No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG1233680.2012731101764548No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA1223850.19966935987407933No Hit
CGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGAT1192260.1945154970000162No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG1184780.19329514580349857No Hit
GGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTA1184400.19323314935233857No Hit
GCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGG1146150.18699271709741883No Hit
CCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGT1129660.18430239741418675No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTT1126220.1837411663826332No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC1089680.1777797181579334No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA1073730.17517749869477076No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCT1006040.16413397296050888No Hit
CCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG1005720.16408176542268993No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC995890.16247801512031448No Hit
TGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT994250.1622104514889924No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC981550.16013846483180338No Hit
GCCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGA976100.15924930520332461No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC967900.15791148704671437No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT966730.1577206032365639No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG966170.15762924004538076No Hit
CCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGAC965840.15757540102200498No Hit
GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCAT964160.15730131144845555No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA960190.15665361168238937No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT953360.15553930704706645No Hit
CCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCC901080.1470099005558977No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC879570.14350057512313108No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA877940.14323464297736588No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA856950.139810154793555No Hit
GGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG841240.1372470909837566No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG836310.1364427686042336No Hit
CCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAG834770.13619151982847996No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG828020.13509026707761176No Hit
CCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGG811870.13245541790331233No Hit
GTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGC810590.13224658775203657No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA802920.13099523832993895No Hit
GCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCC789290.1287715235159636No Hit
CCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGC788280.1286067434747226No Hit
CGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCA781920.12756911866057125No Hit
CACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGG772600.1260485741215947No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA760940.12414626196231722No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC753270.12289491254021959No Hit
CCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGC746940.12186218218273874No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGA746650.12181486910159034No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC740780.12085718707972422No Hit
TCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG739500.12064835692844847No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC737230.1202780097070454No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC735470.11999086824904125No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT733350.1196449933109908No Hit
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGT729620.11903644919828882No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT726770.11857147581458892No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC722340.11784872771290801No Hit
CCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCT719520.11738864878587862No Hit
CCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACT712750.11628413306389676No Hit
GCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAG704670.11496589273396862No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC682430.11133746885555251No Hit
CCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCC680350.11099811985972943No Hit
CCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGG673330.10985281699882653No Hit
CCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTAC670070.10932095270729611No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC665910.10864225471564992No Hit
GGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCA660170.10770578200602277No Hit
CCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGT659380.10757689464703227No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG656340.10708092303775238No Hit
CCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGCC652880.10651642903508511No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT640300.10446402020457816No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG637040.10393215591304776No Hit
CCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACC633730.10339213419373312No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA633100.1032893506036521No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG631300.10299568320342059No Hit
CCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGG630610.1028831106999985No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC629490.10270038431763222No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA625210.10200210849930395No Hit
GTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCC622830.10161381493677563No Hit
CGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCG621260.10135767170435146No Hit
GGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGG618400.1008910668350947No Hit
CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTC617080.10067571074159161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)