FastQCFastQC Report
Thu 6 Oct 2022
EGAF00006436335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006436335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105102502
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA12084691.1498004110311286No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT6745400.6417925236451555No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC5070710.48245378592414484No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT4665660.44391521716581017No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC4136850.3936014767755005No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA4127500.3927118690285793No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT3924950.3734402060190727No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT3840060.36536332883873684No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC3703760.3523950362285381No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG3671190.34929615662241803No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG3527150.3355914400591529No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA3521190.3350243745862491No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG3447020.32796745409543154No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA3290680.3130924514052006No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG3228860.30721057430202753No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC3154080.30009561523092954No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCT3089100.29391307925286114No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC3022960.2876201748270465No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG3002010.28562688260266156No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC2939350.27966508352008596No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA2931710.27893817408837707No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT2924880.27828833227966354No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC2901970.2761085554366727No Hit
CCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGAC2726940.2594552887047351No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGA2704510.25732118156426No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG2703530.257227939254957No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT2554270.24302656467683328No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG2501830.23803714967698866No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT2469830.23499250284260598No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG2432630.23145310089763613No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC2381660.22660354936174595No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA2368060.22530957445713326No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC2333020.22197568617348426No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC2328260.22152279495686983No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG2294950.21835350789270458No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC2264180.2154258896710185No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT2243090.21341927711673314No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA2187260.20810731984287112No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC2181010.20751266225803072No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACC2168590.20633095870543597No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG2154900.20502842073160163No Hit
GGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG2154290.2049703821513212No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC2127680.20243856801810484No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG2121690.20186864818879385No Hit
GGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCAC2093570.19919316478308005No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC2068290.1967878937839177No Hit
GTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT2065560.19652814735085944No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC2062600.19624651751867905No Hit
CCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTAC2057590.19576983999867104No Hit
GCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATC2049070.19495920277901663No Hit
GTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAA2040950.194186623644792No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT2020140.19220665175030752No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG2009680.1912114328163187No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC1961800.18665587999037359No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA1937790.18437144341245082No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG1911380.18185865832194936No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTT1891940.18000903537006188No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC1887970.17963130887217127No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC1879030.17878071066281562No Hit
CCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCC1876260.17851715842121435No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA1845860.17562474392855082No Hit
CTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCA1835460.17463523370737644No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT1826360.17376941226384887No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG1812750.17247448590710046No Hit
CCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG1807590.17198353660505628No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG1776920.1690654329047276No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACC1760170.16747175057735544No Hit
CTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCC1736000.16517209076526077No Hit
CCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGA1731160.16471158793156038No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATA1729320.16453652073858338No Hit
CGCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACG1710650.1627601596011482No Hit
GTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTA1700950.16183725102947596No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1695020.16127303991297942No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC1656700.15762707532880615No Hit
CGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGG1648960.15689065137573985No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC1631050.15518660060062128No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC1621700.1542969928537001No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1618360.1539792078403614No Hit
GGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCG1587500.1510430265494536No Hit
GGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATC1570150.14939225709393675No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA1558480.1482819124515228No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCG1498050.14253228719521824No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT1446280.1376066194884685No Hit
CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTC1445460.13752860041333745No Hit
CCGCCGTTTACCCGCGCTTCATTGAATTTCTTCACTTTGACATTCAGAGC1438860.13690064200374602No Hit
GCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTAT1394450.1326752430689043No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG1389330.1321880995754031No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC1371430.13048500025242027No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC1359630.12936228673224162No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA1357780.12918626808712888No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA1350160.1284612615596915No Hit
TCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG1349680.12841559185717577No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT1314800.12509692680769863No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT1300330.12372017556727623No Hit
CCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGG1296780.12338241005908689No Hit
CCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGG1294850.12319877979688819No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAG1293000.12302276115177543No Hit
TCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCC1269190.12075735361656756No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCT1261590.12003424999340168No Hit
CTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTC1258970.1197849695338366No Hit
CTCGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACAT1257880.11968126125104044No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG1257660.11966032930405404No Hit
CGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAA1243110.11827596644654569No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC1238460.11783354120342443No Hit
GCCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGA1206450.11478794291690601No Hit
CCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACC1204750.11462619605382943No Hit
CGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACC1194620.11366237504032016No Hit
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC1189630.1131876004245836No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT1173250.11162912182623398No Hit
CCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGT1158410.11021716685678899No Hit
CTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACAC1158360.11021240959611028No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA1158270.11020384652688858No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG1153310.10973192626755927No Hit
CGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGAT1145960.10903260894778699No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC1126680.10719820923007141No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGG1122210.1067729101253936No Hit
CGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCG1105020.10513736390404863No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCG1101670.1048186274385742No Hit
CTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGC1096810.10435622170060233No Hit
GCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGA1096470.10432387232798701No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG1087060.10342855586825135No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC1078750.1026378991434476No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT1076610.10243428838639826No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA1074680.10225065812419956No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA1073420.10213077515509572No Hit
CACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGC1055600.10043528744919888No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC1051310.10002711448296445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)