FastQCFastQC Report
Thu 6 Oct 2022
EGAF00006436361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006436361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34348768
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC2210860.6436504505780236TruSeq Adapter, Index 5 (100% over 50bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1327230.386398138064224No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT674530.19637676670091922No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT547140.1592895558874193No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA541950.15777858466423017No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG535060.15577269030435095No Hit
CACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGC524390.15266631979347847No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT521540.1518365957113804No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC490680.14285228512417097No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC447060.13015313969921716No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA424290.1235240809801388No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG412490.12008873214899585No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG396920.11555581847942843No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC385020.11209135652259786No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG370850.10796602661265756No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT365060.1062803766353425No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC364420.10609405263094153TruSeq Adapter, Index 5 (100% over 50bp)
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA355690.10355247675840949No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC353730.10298185949493151No Hit
CGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAA343640.10004434511304743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)