FastQCFastQC Report
Sat 22 Oct 2022
EGAF00006436375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006436375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53338770
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA7759771.45480857545084No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC3746390.7023765264928307TruSeq Adapter, Index 12 (100% over 50bp)
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG2691140.5045373187270723No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC2277090.42691085677453755No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC2127860.39893308375877434No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT2083950.39070079793741025No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC1970120.3693598483804557No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT1686910.3162633859010997No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT1682590.31545346846205863No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT1558330.29215709323630823No Hit
GTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT1429950.2680882967492501No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG1388670.26034908566508No Hit
GGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCAC1322130.2478741073331837No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG1268770.2378701271139173No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA1263060.23679961123962925No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA1254500.23519477483264048No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC1243250.23308561483513773No Hit
GTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAA1237240.23195885469424962No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG1210830.22700748442455648No Hit
CGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCA1106100.20737261095447085No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC1100670.2063545897290095No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1092410.20480599758862084No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACC1056480.19806980925881867No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA1029920.1930903168558255No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT1019790.1911911354536297No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT1015840.1904505859433954No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT995400.18661847657904373No Hit
GGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG979210.18358316099152644No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC954570.1789636318947737No Hit
GCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATC943770.17693883829717108No Hit
GTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTA936160.1755121087344159No Hit
GCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTAT915540.17164625281010415No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC908740.17037138276716918No Hit
GCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAG907490.17013703165633554No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA897760.16831284260960647No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC892440.1673154442818985No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT891000.16704547180221818No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC888430.1665636459183442No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG861200.16145854131994422No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG847240.15884130811415412No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG846970.15879068827421405No Hit
GGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCG844930.15840822726133355No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATA838400.15718397705833861No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG833650.15629344283717078No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT827090.15506356820751582No Hit
CTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCA797790.14957037816957533No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC790840.1482673859933403No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC789880.14808740434022008No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG780170.14626696491126437No Hit
GGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA756780.1418817869253453No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA749860.14058441917577028No Hit
GTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGC744880.13965076435020907No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT721350.13523933903987662No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA704870.13214965399464593No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT698050.1308710343339376No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA674750.12650272962799855No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT668100.12525598171836358No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC662130.12413672081302211No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC653590.1225356340238067No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA652320.12229753329519973No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT645760.1210676586655448No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG629460.11801172018027413No Hit
GGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTGCTGCGTGCTTGAT618770.11600754948042484No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC614740.11525200149909717No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG608970.11417023677148912No Hit
CCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGG608600.11410086884268236No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA602590.11297410870179422No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG602480.11295348580404084No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC592410.11106555325516504No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG591220.11084245099765144No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG590910.11078433192216468No Hit
CCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGAC590890.11078058230439135No Hit
TGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT584070.10950196264368302No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC578470.10845206966714832No Hit
GTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAG577000.10817647276080795No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT560890.10515615564438399No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA558030.10461996030279663No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAG546690.10249392702531386No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC541660.10155089815531929No Hit
CCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCC538060.10087596695611842No Hit
CCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG534480.10020478537469087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)