FastQCFastQC Report
Thu 6 Oct 2022
EGAF00006436425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006436425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101664243
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA6967940.6853874867292329No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT6206670.6105066852265846No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT5427240.5338396116321842No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG4899440.4819236198906237No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA4623110.45474297192179947No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA4034840.3968789695311064No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA3234120.31811774765292844No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT3008030.2958788568366166No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG2965180.29166400225888667No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG2916410.2868668387173256No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG2897260.2849831872549329No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC2795070.27493147221880165No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC2770360.2725009224728108No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT2731610.26868935619773415No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA2554080.2512269726928474No Hit
CGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCAT2515680.2474498334679972No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC2507880.2466826020629495No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC2500500.24595668311817362No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT2485920.2445225505687383No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG2482420.24417828006647332No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG2462320.24220118375346578No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA2422690.23830305803781965No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC2406150.23667613400711593No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT2363900.2325202972297743No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC2347770.23093370202933594No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC2338670.23003859872344692No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA2223760.2187357063190841No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG2188750.2152920176664277No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC2128260.20934203975728222No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG2073180.20392420568163772No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC2072180.20382584268099058No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC2066060.2032238611170301No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG2024990.19918409268045206No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG2024590.1991447474801932No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC1989990.1957413876578022No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG1942730.19109275224721833No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG1929340.18977567166855314No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA1926600.18950615704677995No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT1877840.1847099771352254No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA1862100.18316174350503944No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA1807270.17776850017955673No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATA1791130.1761809213491119No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT1765550.17366479579255806No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG1743620.17150769518836628No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT1740210.17117227735615953No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA1676580.164913439624982No Hit
CCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGAC1660550.16333668072460836No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC1659150.16319897252370236No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC1656870.1629747048822269No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCT1652630.162557645759483No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG1648910.16219173539707565No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA1641220.16143532392209914No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT1639070.16122384347070778No Hit
CCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGT1633860.1607113722373362No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCG1607980.1581657377805882No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG1583050.155713548174455No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA1571870.15461384982721998No Hit
GGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATC1569930.15442302560596452No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG1569320.15436302417556977No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT1547670.1522334652115592No Hit
GTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAA1536740.15115835761448593No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAG1535110.15099802592343112No Hit
GTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT1524280.14993275462642258No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC1512270.14875141498865044No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTT1489410.1465028367938568No Hit
CACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTG1481420.14571691641868617No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC1458450.14345751829382136No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC1453000.14292143994029444No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC1451860.14280930611955672No Hit
CTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGT1446370.1422692932460039No Hit
CGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTA1414160.13910102099515953No Hit
CTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTC1414080.13909315195510774No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT1394880.13720458234268265No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC1392360.13695670758105188No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA1382560.1359927501747099No Hit
GCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTAT1379920.13573307185300146No Hit
GCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATC1347920.13258545583229298No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC1343920.13219200382970442No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA1343160.1321172479492126No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC1328300.13065557375959608No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT1325200.13035064845758995No Hit
TCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCT1313190.1291693088198178No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGA1308650.1287227407968798No Hit
CTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTT1306980.12855847458579905No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT1301250.12799485459209095No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGG1291400.1270259790357166No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC1288940.12678400605412465No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA1279380.125843655767938No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACC1277560.1256646351067602No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG1277260.12563512620656606No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG1277070.1256164372364431No Hit
CCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGT1263900.12432099651792028No Hit
CTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCA1252130.12316326400030343No Hit
CACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACT1247700.1227275159074366No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC1243840.12234783472493864No Hit
CCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACC1243170.12228193151450505No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC1240080.12197798984250541No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCT1227370.12072779610428025No Hit
CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTC1226270.12061959680356839No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT1225150.1205094302428436No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA1217950.11980121663818419No Hit
CCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCC1214120.11942448634570564No Hit
GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCAT1213500.11936350128530442No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA1209610.11898086921278703No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC1202700.1183011808783153No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT1197350.1177749388248531No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC1197010.11774149540463308No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC1194520.1174965715330217No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT1190010.11705295440010309No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA1189950.11704705262006426No Hit
GGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCAC1186940.11675097998811637No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA1180110.1160791606936964No Hit
ACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTC1174820.11555882042027305No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC1172590.11533947092882992No Hit
TCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCC1166740.11476404737504413No Hit
GTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTA1166080.11469912779461704No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC1153770.11348827925665075No Hit
GCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTC1150940.11320991196481933No Hit
CCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATA1147480.11286957598258023No Hit
GCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATT1146900.11281252544220488No Hit
CTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAA1139430.11207775382737076No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT1135580.11169905627487926No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC1135280.11166954737468512No Hit
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC1131050.11125347188194772No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC1123650.1105255856771589No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG1123130.11047443691682238No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG1095080.10771535474867008No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG1093070.10751764511736935No Hit
CGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGAT1079060.10613957947830291No Hit
CCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACT1073480.10559071393469185No Hit
CGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGG1067100.10496315799056312No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGG1060630.10432674937637611No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG1058790.10414576145518538No Hit
CGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAA1056480.10391854292369049No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC1056470.10391755929368401No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1056460.10391657566367754No Hit
TGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGC1036090.10191292134049529No Hit
GTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTT1032000.1015106166678485No Hit
CGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCG1031680.10147914050764141No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC1018940.10022599587939685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)