FastQCFastQC Report
Thu 6 Oct 2022
EGAF00006436452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006436452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36006581
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT2699760.7497962664102987Illumina Single End PCR Primer 1 (100% over 50bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1869320.5191606501044906No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG700100.19443667811725862No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG668610.1856910546435942Illumina Single End PCR Primer 1 (100% over 50bp)
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC528390.14674817361859488No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG498360.1384080315762277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)