FastQCFastQC Report
Thu 6 Oct 2022
EGAF00006436551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006436551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48614778
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG4644080.9552815401111161No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1781770.3665078960146645No Hit
GCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGT1502810.30912616735594267No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGG1361270.2800115635620099No Hit
CCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACC1357620.2792607630543947No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA1354640.27864778072214996No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1327200.27300340649503735Illumina Single End PCR Primer 1 (100% over 50bp)
CGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGG1277130.26270406912071054No Hit
CCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCC1221580.25127750249111497No Hit
TGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACC1080440.222245178204866No Hit
GTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTA1079880.22212998689410862No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1029710.2118100796428609No Hit
CGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTC1016800.20915450853236436No Hit
CGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAA1016550.20910308384006196No Hit
GGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCT989620.20356361598524628No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA977090.20098621040704948No Hit
CCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCC946520.19469799903231072No Hit
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT926800.19064161930349655No Hit
GTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAG921710.1895946125682195No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATG872100.1793898966277291No Hit
TGGGGATCGGGGATTGCAATTATTCCCCATGAACGAGGAATTCCCAGTAA811870.16700065975823236No Hit
GGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTC807810.1661655227552412No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG770220.15843330602065075No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC758220.15596492079013505No Hit
CCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATGAACGAGG756370.1555843780670972No Hit
GGCGCGGGTAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCA721720.1484569157139831No Hit
GCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGA694410.14283928232686777No Hit
CCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGA687880.1414960693639288No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT686370.14118546422242226No Hit
ACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACC679530.13977848464102827No Hit
AGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAG675910.13903385509648938No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC674230.13868828116421716No Hit
GGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAA668770.13756516588433254No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG641960.13205038188182203No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA637620.1311576492234522No Hit
TGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAA633770.13036570896199506No Hit
CAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGA629230.12943183654978327No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC581620.1196385181477122No Hit
CGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCC558180.1148169389974382No Hit
CTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATA544850.11207497440387365No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT517270.1064018023490717No Hit
ATCGGGGATTGCAATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCG516650.10627426911216174No Hit
CTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTT514680.10586904253681874No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAA513640.10565511581684071No Hit
GGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAG511590.10523343333996094No Hit
GCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGT510950.10510178612766678No Hit
GCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCC504620.10379971291856974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)