FastQCFastQC Report
Sat 22 Oct 2022
EGAF00006436565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006436565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51050023
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG4348290.8517704291729702No Hit
CCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCC1748060.3424210014557682No Hit
GCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGT1610420.31545921144834743No Hit
CGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAA1491080.2920821406877721No Hit
CCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGA1399510.2741448324127102No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGG1321620.25888724868938845No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC1319620.2584954760941048No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA1268600.24850135718841887No Hit
CGAGGAATTCCCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCC1201660.23538872842427516No Hit
CGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGG1176730.23050528302406445No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG1175510.23026630174094143No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1165720.22834857488702795No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA1160520.22732996613929046No Hit
TGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACC988780.1936884533822835No Hit
CCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATGAACGAGG971200.1902447722697402No Hit
CCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACC952420.18656602760002675No Hit
ATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCAT949670.18602734028151172No Hit
GGAATTCCCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGC945030.18511842786045365No Hit
CGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTC937560.1836551572170692No Hit
GGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCT919870.18018992861178534No Hit
CCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCC905620.17739854887038933No Hit
GAGGAATTCCCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCT888360.17401755137309144No Hit
GTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTA884020.17316740484132592No Hit
GGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGA863540.16915565346562134No Hit
TGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATG858680.16820364605908208No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC832110.16299894713073879No Hit
CAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAA827210.16203910427229384No Hit
TGGGGATCGGGGATTGCAATTATTCCCCATGAACGAGGAATTCCCAGTAA820280.160681612229636No Hit
CTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTT819840.1605954222586736No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG813810.15941422788389342Illumina Single End PCR Primer 1 (100% over 50bp)
CTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGT762570.14937701399272632No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATG759820.1488383266742113No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC756570.14820169620687537No Hit
GGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTC753020.14750629985024688No Hit
GTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAG750240.1469617359428026No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC743340.145610120489074No Hit
ACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACC718220.1406894566923114No Hit
GGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAG712470.1395631104808709No Hit
CAACTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGC706640.13842109336561903No Hit
CCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTC690330.13522618785108087No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT687430.1346581175879196No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA663420.1299548875815394No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG660770.12943578889278853No Hit
GAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTC642430.12584323419403748No Hit
ATCGGGGATTGCAATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCG623810.12219583133194671No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTT618430.12114196305063368No Hit
CGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGA594410.11643677418127707No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG591990.11596272934098384No Hit
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT586240.11483638312954335No Hit
AGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAG577650.11315371983280006No Hit
CAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGA574550.11254647231011042No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCG536280.10504990369935778No Hit
CTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATA533300.10446616253238514No Hit
CGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTG531360.10408614311496No Hit
TGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAA530990.10401366518483254No Hit
CCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG522720.10239368550333465No Hit
CTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAAC511860.10026636031094444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)