FastQCFastQC Report
Mon 13 May 2024
EGAF00006464830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006464830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72048429
Total Bases10.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT3897860.5410055505859815No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT3147380.43684228007247733No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC3133930.4349754801732041No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2990130.4150166827371073No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC2915020.40459175036280115No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2758530.3828716376314048No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA2621000.36378308817809196No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG2508040.34810474493482707No Hit
CCCGCCGCCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGG2382230.3306428791112156No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2357880.3272632079181074TruSeq Adapter, Index 10 (100% over 50bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2225390.30887418794377874No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG2196800.30490602369692194No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG2185080.3032793400672206No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1979210.27470550398815774No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1662640.23076700256712052No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1429540.19841376416410134No Hit
CCCCGCCGCCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGG1406430.19520619943010833No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1341020.18612758371178365No Hit
GTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCG1251750.17373730661080758No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT1236630.17163871817385498No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC1231200.17088505843756843TruSeq Adapter, Index 10 (98% over 50bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1193960.16571631284285185No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT1165480.16176341610446496No Hit
CTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT1098080.15240859727836675No Hit
CTCCCCCCGCCGCCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGA1081140.1500574009740032No Hit
CCCCCCGCCGCCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACG1036530.14386573231180377No Hit
CCCCCGCCGCCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGG1029700.14291775883135496No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC967270.13425275379703283No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT951290.13203480120295197No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC930800.1291908807616055No Hit
CCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG914990.12699652340788722No Hit
TGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA893560.12402213516688892No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC867760.12044121045304125No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT862980.11977776781225861No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC822510.11416071265065335No Hit
CCGCCGCCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGA818330.11358054732879741No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG786330.10913909031937392No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA768190.106621339377157No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)