FastQCFastQC Report
Thu 3 Nov 2022
EGAF00006465111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006465111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46900255
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC19248994.104239944964052TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGC7278701.5519531823441046TruSeq Adapter, Index 12 (98% over 50bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT967680.20632723638709427No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT895070.19084544423052713No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT870980.18570901160345502No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGATGC742640.1583445548430387TruSeq Adapter, Index 12 (98% over 50bp)
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG729330.15550661718150574No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC723750.15431685819192242No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTGC652180.1390568132305464TruSeq Adapter, Index 12 (98% over 50bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG605660.12913789061488046No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATTC570990.1217456067136522TruSeq Adapter, Index 12 (98% over 50bp)
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG538540.11482666778677428No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA525830.11211666119939007No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)