FastQCFastQC Report
Wed 10 Jul 2024
EGAF00006465125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006465125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95333165
Total Bases14.1 Gbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3221750.3379464009193443No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2672020.2802823130859025No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2132300.22366822710648493No Hit
GCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCT1483860.15564992518605672No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1455890.15271600392161533No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1217630.12772365209945563No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1179200.12369252610043943No Hit
CACCGTTCGGCCTCGGGCACCTGAGGGACAACCCGGAGCGCTCCAGGAGC1095610.1149243287999512No Hit
GGCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACC1019520.10694284617530532No Hit
GGGACCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCGC973110.10207465576119287No Hit
CTGGAGGCGACAGCGAGGGCTGTCTGCCGCGTCAGAGGACCCCGCCGGCC965250.10125017878091008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)