FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2303111
Sequences flagged as poor quality0
Sequence length36-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC105320.4572945029570872Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC103660.45008686077223375Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCCTCTCCGGAATCGAACCCCGATTCCCCGTTACCCGTAGTAACCACG54860.2381995483500361No Hit
CATGCTCCCTCTCCGGAATCGAACCCCGATTCCCCGTTACCCGTAGTAAC42670.18527113977572077No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT41580.18053841087120856TruSeq Adapter, Index 1 (97% over 35bp)
CTGCCTTCCTTGGATGTGGTAGCCGTTTCGCATGCTCCCTCTCCGGAATC38650.16781648821962988No Hit
CCTTCCTTGGATGTGGTAGCCGTTTCGCATGCTCCCTCTCCGGAATCGAA35740.15518140463051933No Hit
GTAGCCGTTTCGCATGCTCCCTCTCCGGAATCGAACCCCGATTCCCCGTT34750.1508828710383477No Hit
CCCTATCAACTGTCGACGGTCGGTTAAGTGCCTACCGTGGTTACTACGGG32710.1420252866666001No Hit
AACCCCGATTCCCCGTTACCCGTAGTAACCACGGTAGGCACTTAACCGAC31160.13529525932532127No Hit
CTACGGGTAACGGGGAATCGGGGTTCGATTCCGGAGAGGGAGCATGCGAA27910.12118391167425278No Hit
GGTTCGATTCCGGAGAGGGAGCATGCGAAACGGCTACCACATCCAAGGAA27470.11927345229995427No Hit
CGGGTAACGGGGAATCGGGGTTCGATTCCGGAGAGGGAGCATGCGAAACG27260.11836164214403908No Hit
CTTAACCGCCCTATCAACTGTCGACGGTCGGTTAAGTGCCTACCGTGGTT26060.11315129839595227No Hit
CACTTAACCGCCCTATCAACTGTCGACGGTCGGTTAAGTGCCTACCGTGG25620.11124083902165376No Hit
TTTCGCATGCTCCCTCTCCGGAATCGAACCCCGATTCCCCGTTACCCGTA24780.10759359839799297No Hit
CCCCGATTCCCCGTTACCCGTAGTAACCACGGTAGGCACTTAACCGACCG24690.10720282261688648No Hit
GTTCGATTCCGGAGAGGGAGCATGCGAAACGGCTACCACATCCAAGGAAG24550.10659494917960967No Hit
ATGTGGTAGCCGTTTCGCATGCTCCCTCTCCGGAATCGAACCCCGATTCC24460.10620417339850315No Hit
GGGTTCGATTCCGGAGAGGGAGCATGCGAAACGGCTACCACATCCAAGGA23590.10242667418114021No Hit
TAGCCGTTTCGCATGCTCCCTCTCCGGAATCGAACCCCGATTCCCCGTTA23370.10147144449399095No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)