FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2303111
Sequences flagged as poor quality0
Sequence length36-150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCTCTCCGGAATCGAACCCCGATTCCCCGTTACCCGTAGTAACCACG46870.2035073428940246No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG41740.18123312337095349Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG39660.17220186087426964Illumina Single End PCR Primer 1 (100% over 32bp)
CATGCTCCCTCTCCGGAATCGAACCCCGATTCCCCGTTACCCGTAGTAAC37700.16369163275239448No Hit
GTAGCCGTTTCGCATGCTCCCTCTCCGGAATCGAACCCCGATTCCCCGTT33840.14693169369604853No Hit
CTGCCTTCCTTGGATGTGGTAGCCGTTTCGCATGCTCCCTCTCCGGAATC33250.14436994135323916No Hit
CCTTCCTTGGATGTGGTAGCCGTTTCGCATGCTCCCTCTCCGGAATCGAA31830.13820436791800308No Hit
CCCTATCAACTGTCGACGGTCGGTTAAGTGCCTACCGTGGTTACTACGGG30960.13442686870064013No Hit
AACCCCGATTCCCCGTTACCCGTAGTAACCACGGTAGGCACTTAACCGAC29300.12721922651578668No Hit
CGGGTAACGGGGAATCGGGGTTCGATTCCGGAGAGGGAGCATGCGAAACG27960.12140100933042307No Hit
GGTTCGATTCCGGAGAGGGAGCATGCGAAACGGCTACCACATCCAAGGAA24970.10841856949144006No Hit
CTTAACCGCCCTATCAACTGTCGACGGTCGGTTAAGTGCCTACCGTGGTT24960.108375149960206No Hit
CTACGGGTAACGGGGAATCGGGGTTCGATTCCGGAGAGGGAGCATGCGAA24010.10425029449297059No Hit
CACTTAACCGCCCTATCAACTGTCGACGGTCGGTTAAGTGCCTACCGTGG24010.10425029449297059No Hit
TTTCGCATGCTCCCTCTCCGGAATCGAACCCCGATTCCCCGTTACCCGTA23440.10177538121262934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)