FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006469155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3055548
Sequences flagged as poor quality0
Sequence length36-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAG2078396.802020455905128No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG1264984.139944782408916No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG802892.6276464974531573No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC687452.2498419268818557No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC642532.102830654272163No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC283610.9281804769553611No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC235450.7705655417620669No Hit
GAGTATGCCTGCCGTGTGAACCATGTGACTTTGTCACAGCCCAAGATAGT130990.4286956055018608No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC129120.4225755903687326No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC111200.36392817262239047No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC95760.3133971385820154No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC95280.31182622560666695No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC90710.2968698249872036No Hit
CCCACTGTGTTACTGCCATCGACTTACATTGGTGGTCTTTTTGTAATAGT89650.29340072549997576No Hit
AGGAAACACAATTAGCCTGGACAATAAATGAACACTTACAGTAGCAGTTC73280.2398260475698631No Hit
CCTGAGAGTGGAGCTGGAGAGACTGAGAGGGGCTCAGAGCCCTAGCGCCT72630.23769876958241207No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC72500.23727331398492188No Hit
GGTGCCCTACTACCTGGAGAACGAGCCCAGCGGCTACACGGTGCGCGAGG65800.21534598703734975No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT55400.1813095392381334No Hit
TGTTCTGGCGGTGTTTTGAAATTAGTTATTTCTCTTGCTTCTTTCTTGAT50960.16677859421616023No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC49240.1611494893878283No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC48950.1602003961318886No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAA45050.14743672820718248No Hit
GCTTCTTGGTCGTGTTCTTCATTCGGCACAGGATGACTGTTACCACCATA44830.14671672642681444No Hit
CTCTCCACGCTCCCTCCACTCCCTCTGCAGGGCCCCTGTACGTGATCGTG42720.13981125480601186No Hit
GCATCAAAGCAGAGGACAAGATCCACAAGCTGGCTGGGTCATGGGGGAGG40520.1326112370023315No Hit
CCTTCTCCGCACATTTTACAAGATGGCTTGTCCCAGTGGTGGATGTAGAT39570.12950213840528768No Hit
TCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCCTCTCCCTCCCTCC38140.12482212683289544No Hit
GTGGCGGAGGGACTGGGGACCCGGGCACCCGTCCTGCCCCTTCACCTTCC33170.10855663206730838No Hit
GCCCAGGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGC32780.10728026527483778No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACCGTCAAACTT32670.10692026438465375No Hit
TCACATCGTCCCCCTTTTTAGGTAGATGAAGGATAGTCACATATTTGTTA30910.10116025014170944No Hit
AATTGCCCCGCAGCCACCCACCTCCCGCCCGCAGGTTGGAGGCAGGCGCC30790.10076752189787233No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)