FastQCFastQC Report
Mon 25 Jul 2022
EGAF00006469160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266484
Sequences flagged as poor quality0
Sequence length36-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC17980.6747121778418216Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC12260.46006514462406745Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT10250.3846384773569895TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG4590.1722429864457153Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)