FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006469167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5722762
Sequences flagged as poor quality0
Sequence length36-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAG5065228.85100586045689No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG3101095.418869420045775No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG1973563.448614497684859No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC1707912.9844155671684405No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC1593802.785018842300274No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC697931.2195684531350421No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC604531.0563605475817446No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC325410.5686240315428109No Hit
GAGTATGCCTGCCGTGTGAACCATGTGACTTTGTCACAGCCCAAGATAGT307950.5381142881706421No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC276940.48392716663736846No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC236210.4127552395154647No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC232650.4065344670982299No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC225460.39397060370499426No Hit
CCCACTGTGTTACTGCCATCGACTTACATTGGTGGTCTTTTTGTAATAGT206860.36146881523292423No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC187430.32751667813548774No Hit
CCTGAGAGTGGAGCTGGAGAGACTGAGAGGGGCTCAGAGCCCTAGCGCCT179520.31369468099494613No Hit
AGGAAACACAATTAGCCTGGACAATAAATGAACACTTACAGTAGCAGTTC178010.31105609494156844No Hit
GGTGCCCTACTACCTGGAGAACGAGCCCAGCGGCTACACGGTGCGCGAGG165760.2896503471575439No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT138620.24222569451603965No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC124650.21781440500233976No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC122280.21367304808412443No Hit
TGTTCTGGCGGTGTTTTGAAATTAGTTATTTCTCTTGCTTCTTTCTTGAT116500.20357302994602955No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAA109020.19050241823790678No Hit
CTCTCCACGCTCCCTCCACTCCCTCTGCAGGGCCCCTGTACGTGATCGTG108980.19043252191861204No Hit
GCTTCTTGGTCGTGTTCTTCATTCGGCACAGGATGACTGTTACCACCATA106930.18685033555475486No Hit
TCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCCTCTCCCTCCCTCC98600.17229442706161816No Hit
GCATCAAAGCAGAGGACAAGATCCACAAGCTGGCTGGGTCATGGGGGAGG97890.1710537673941359No Hit
CCTTCTCCGCACATTTTACAAGATGGCTTGTCCCAGTGGTGGATGTAGAT92610.16182745324722572No Hit
GTGGCGGAGGGACTGGGGACCCGGGCACCCGTCCTGCCCCTTCACCTTCC86030.1503295087232354No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACCGTCAAACTT80850.14127793537456215No Hit
GCCCAGGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGC79770.13939073475360325No Hit
AATTGCCCCGCAGCCACCCACCTCCCGCCCGCAGGTTGGAGGCAGGCGCC76460.13360681433196067No Hit
TCACATCGTCCCCCTTTTTAGGTAGATGAAGGATAGTCACATATTTGTTA76240.13322238457583943No Hit
CCCGAGACCCCCAGCGCTACCTTGTCATTCAGGTACAAATTGCAGTCTGT74100.12948293149356901No Hit
GACTAGTTTGGAACCTGCAGCCGATTCCTGGCTTTTCATCTCTTTCAATT72830.12726372335595995No Hit
CTTTCCATTCTCTGCTGGATGACGTGAGTAAACCTGAATCTTTGGAGTAC71470.12488724849993763No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGAACAATAAGGCTG69300.12109537317819613No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGATGGTGAGTCTT68670.11999450614930345No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTCACAGATAACTCC64180.11214864430846505No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCACTTTTAGCTGA63460.11089051056115909No Hit
CCCATCCAGTTCTGCCCCATCTCCCTCACGTTGCTGGTTTTCTTATAGTA63070.11020902144803506No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACAGAGAGTTTA62610.10940521377614516No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTCACAGTGAGCGTA61800.10798981331042598No Hit
CCGCGGAAAGGAAGGGGAGGGATGGAGAGGCCAGCTGCAGCTATGGAGTC61320.1071510574788887No Hit
GAGGGGTGCAGCATTGGTGAACATTCTTTTGAACATGAACCTGAAAACTC60980.10655693876488312No Hit
GAAGGATAGGACAGGGTGGGCTGGGCCAGGCTGCATGCGCAGAGGGACAG60590.10587544965175906No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAT58020.10138461113706984No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)