FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845852
Sequences flagged as poor quality0
Sequence length36-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC143351.6947409239441416Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC133711.5807729957486651Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT39580.4679305599561152TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC26580.3142393704808879Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG26370.31175666665090346Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)