FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006469184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267927
Sequences flagged as poor quality0
Sequence length36-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC196971.5534806025898966Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC154781.220732739345404Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT66570.5250302265035763TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG55850.4404827722731671Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC40300.31784164230275086Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)