FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267927
Sequences flagged as poor quality0
Sequence length36-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG96420.7604538747104526Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG74180.5850494547399022Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG31760.25048760693636146Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG27640.21799362266124153Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT26190.20655763304985225Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT24550.1936231344549016Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG22310.1759565022276519Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG18380.14496102693609333Illumina Single End PCR Primer 1 (100% over 35bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTG16320.12871403479853336Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)