FastQCFastQC Report
Wed 20 Jul 2022
EGAF00006469192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965011
Sequences flagged as poor quality0
Sequence length36-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC107161.1104536632224917Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC106151.0999874612828249Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT33760.34984057176550315TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC20540.21284731469382212Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG16530.17129338422049073Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)