FastQCFastQC Report
Wed 20 Jul 2022
EGAF00006469193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965011
Sequences flagged as poor quality0
Sequence length36-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG43640.4522228244030379Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG43320.4489068000261137Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG14250.14766671053490582Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT11670.12093126399595445Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT10930.11326295762431723Illumina Single End PCR Primer 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)