FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006469205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791078
Sequences flagged as poor quality0
Sequence length36-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG85000.4745745299758023Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG78950.440795989901054Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG26760.1494072284959114Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT21850.12199357035260329Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT19490.10881714810856925Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG18710.10446222889232072Illumina Single End PCR Primer 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)