FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4623243
Sequences flagged as poor quality0
Sequence length36-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAG46617010.083181870388383No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG2832426.126478750954687No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG1814983.925772450204326No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC1545783.3434971945017815No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC1442513.1201258510530376No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC633741.370769392826637No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC545011.1788478347341897No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC292810.6333433046889381No Hit
GAGTATGCCTGCCGTGTGAACCATGTGACTTTGTCACAGCCCAAGATAGT287260.621338744253763No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC248380.5372419316916719No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC213580.46197009328733096No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC209440.45301534009784905No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC205150.4437361393290381No Hit
CCCACTGTGTTACTGCCATCGACTTACATTGGTGGTCTTTTTGTAATAGT193650.4188618249138105No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC166940.3610885259546167No Hit
AGGAAACACAATTAGCCTGGACAATAAATGAACACTTACAGTAGCAGTTC162680.3518742147016715No Hit
CCTGAGAGTGGAGCTGGAGAGACTGAGAGGGGCTCAGAGCCCTAGCGCCT161680.3497112308394778No Hit
GGTGCCCTACTACCTGGAGAACGAGCCCAGCGGCTACACGGTGCGCGAGG146650.3172015833907065No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT125150.2706974303535419No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC112930.24426576755753482No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC111780.24177833611601207No Hit
TGTTCTGGCGGTGTTTTGAAATTAGTTATTTCTCTTGCTTCTTTCTTGAT110400.23879341838618476No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAA101170.2188290773381369No Hit
GCTTCTTGGTCGTGTTCTTCATTCGGCACAGGATGACTGTTACCACCATA100480.21733661847322325No Hit
CTCTCCACGCTCCCTCCACTCCCTCTGCAGGGCCCCTGTACGTGATCGTG97230.2103069209210937No Hit
CCTTCTCCGCACATTTTACAAGATGGCTTGTCCCAGTGGTGGATGTAGAT88240.1908616959999723No Hit
GCATCAAAGCAGAGGACAAGATCCACAAGCTGGCTGGGTCATGGGGGAGG88040.19042909922753357No Hit
TCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCCTCTCCCTCCCTCC87890.19010465164820453No Hit
GTGGCGGAGGGACTGGGGACCCGGGCACCCGTCCTGCCCCTTCACCTTCC75770.16388928723841684No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACCGTCAAACTT75590.16349995014322197No Hit
GCCCAGGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGC72990.15787619210151835No Hit
TCACATCGTCCCCCTTTTTAGGTAGATGAAGGATAGTCACATATTTGTTA69850.15108442277423012No Hit
AATTGCCCCGCAGCCACCCACCTCCCGCCCGCAGGTTGGAGGCAGGCGCC69020.14928914616860936No Hit
CCCGAGACCCCCAGCGCTACCTTGTCATTCAGGTACAAATTGCAGTCTGT67910.14688823408157434No Hit
GACTAGTTTGGAACCTGCAGCCGATTCCTGGCTTTTCATCTCTTTCAATT66190.14316790183860117No Hit
CTTTCCATTCTCTGCTGGATGACGTGAGTAAACCTGAATCTTTGGAGTAC64850.1402695034632616No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGATGGTGAGTCTT64300.13907986233905506No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCACTTTTAGCTGA60390.13062259543787771No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGAACAATAAGGCTG60370.13057933576063382No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTCACAGATAACTCC59750.1292382857660737No Hit
CCCATCCAGTTCTGCCCCATCTCCCTCACGTTGCTGGTTTTCTTATAGTA58150.1257775115865638No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTCACAGTGAGCGTA57890.12521513578239346No Hit
GAGGGGTGCAGCATTGGTGAACATTCTTTTGAACATGAACCTGAAAACTC56150.1214515438621764No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACAGAGAGTTTA55980.12108383660560347No Hit
GAAGGATAGGACAGGGTGGGCTGGGCCAGGCTGCATGCGCAGAGGGACAG54990.1189424825820317No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAT53750.11626038259291152No Hit
CCGCGGAAAGGAAGGGGAGGGATGGAGAGGCCAGCTGCAGCTATGGAGTC50970.11024728745601302No Hit
ACTCACACTCACAAGCCTCACAGCCAGGCCTTCATTTATACCCCAAACAC50510.10925231487940391No Hit
GAGGGCCCACAGAGGAGGACTCACGGGGACACTTCTTCACGCAGGTGGCA49230.10648369553579598No Hit
TGCTAAGTGGCTAGGGACGTAGGGCTATATAATACCAGAAAACTCAATGA48220.10429908183498035No Hit
CACTGTCACCCCTTCCTTGGCACTTTTTTGATTCTTTAATCTTCTATGCC48200.10425582215773646No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTTACTTGTAAAGTT47430.10259032458384731No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAA47000.10166024152310402No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACTGTCAGTCTG46950.10155209232999435No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGCACTTGGAGTCTT46430.1004273407216536No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)