FastQCFastQC Report
Fri 22 Jul 2022
EGAF00006469220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199614
Sequences flagged as poor quality0
Sequence length36-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC19120.9578486478904285Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT13920.6973458775436593TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC13010.6517578927329747Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG4800.24046409570471008Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC2040.1021972406745018Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)