FastQCFastQC Report
Mon 25 Jul 2022
EGAF00006469221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199614
Sequences flagged as poor quality0
Sequence length36-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG8650.4333363391345296Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG6180.30959752321981426Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG5280.2645105052751811Illumina Single End PCR Primer 1 (100% over 32bp)
CATGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCC3950.19788191209033434No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT2220.1112146442634284Illumina Single End PCR Primer 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)