FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006469223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140818
Sequences flagged as poor quality0
Sequence length36-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAG1864968.711436469611149No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG1161165.423908057574255No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG752773.5162727518172963No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC635662.9692388610334928No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC592602.768100791379744No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC257451.202577706278628No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC222451.0390887968991291No Hit
GAGTATGCCTGCCGTGTGAACCATGTGACTTTGTCACAGCCCAAGATAGT120160.5612807814583023No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC118460.5533398915741553No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC101940.47617312634703185No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC87220.4074143621737112No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC87170.40718080658888334No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC84800.396110271868043No Hit
CCCACTGTGTTACTGCCATCGACTTACATTGGTGGTCTTTTTGTAATAGT81420.38032191433367996No Hit
AGGAAACACAATTAGCCTGGACAATAAATGAACACTTACAGTAGCAGTTC67930.3173086175471245No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC66740.31174999462822156No Hit
CCTGAGAGTGGAGCTGGAGAGACTGAGAGGGGCTCAGAGCCCTAGCGCCT66410.3102085277683577No Hit
GGTGCCCTACTACCTGGAGAACGAGCCCAGCGGCTACACGGTGCGCGAGG58740.2743811010557647No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT50440.23561087397434066No Hit
TGTTCTGGCGGTGTTTTGAAATTAGTTATTTCTCTTGCTTCTTTCTTGAT46040.21505798250948935No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC44780.2091723817718274No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC44600.20833158166644714No Hit
GCTTCTTGGTCGTGTTCTTCATTCGGCACAGGATGACTGTTACCACCATA42120.1967472246589855No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAA39990.18679775674531884No Hit
GCATCAAAGCAGAGGACAAGATCCACAAGCTGGCTGGGTCATGGGGGAGG39020.18226677839965844No Hit
CTCTCCACGCTCCCTCCACTCCCTCTGCAGGGCCCCTGTACGTGATCGTG38210.17848317792544718No Hit
CCTTCTCCGCACATTTTACAAGATGGCTTGTCCCAGTGGTGGATGTAGAT35500.1658244652277774No Hit
TCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCCTCTCCCTCCCTCC35150.16418957613398244No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACCGTCAAACTT31270.14606566275134084No Hit
GCCCAGGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGC31060.14508472929506386No Hit
GTGGCGGAGGGACTGGGGACCCGGGCACCCGTCCTGCCCCTTCACCTTCC30510.14251561786195743No Hit
TCACATCGTCCCCCTTTTTAGGTAGATGAAGGATAGTCACATATTTGTTA29300.13686357270912333No Hit
GACTAGTTTGGAACCTGCAGCCGATTCCTGGCTTTTCATCTCTTTCAATT28030.1309312608544958No Hit
AATTGCCCCGCAGCCACCCACCTCCCGCCCGCAGGTTGGAGGCAGGCGCC27580.1288292605910451No Hit
CCCGAGACCCCCAGCGCTACCTTGTCATTCAGGTACAAATTGCAGTCTGT27400.12798846048566484No Hit
CTTTCCATTCTCTGCTGGATGACGTGAGTAAACCTGAATCTTTGGAGTAC26950.1258864602222141No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGATGGTGAGTCTT26580.124158148894488No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGAACAATAAGGCTG26170.12224299309889959No Hit
CCCATCCAGTTCTGCCCCATCTCCCTCACGTTGCTGGTTTTCTTATAGTA24510.11448894768261479No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCACTTTTAGCTGA24470.1143021032147525No Hit
CCGCGGAAAGGAAGGGGAGGGATGGAGAGGCCAGCTGCAGCTATGGAGTC24210.11308761417364764No Hit
GAGGGGTGCAGCATTGGTGAACATTCTTTTGAACATGAACCTGAAAACTC24120.11266721412095751No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTCACAGATAACTCC23900.11163956954771495No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTCACAGTGAGCGTA23580.11014481380481665No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACAGAGAGTTTA22990.10738885790384797No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAT22610.10561383545915626No Hit
CACTGTCACCCCTTCCTTGGCACTTTTTTGATTCTTTAATCTTCTATGCC22390.1045861908859137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)