FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006469227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3519727
Sequences flagged as poor quality0
Sequence length36-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAG43252112.288481464613591No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG2613337.424808799091521No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG1660044.716388515359288No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC1412324.012583930515066No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC1330313.7795829051514502No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC590791.6785108617799052No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC504661.4338043831240321No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC276980.7869360322547743No Hit
GAGTATGCCTGCCGTGTGAACCATGTGACTTTGTCACAGCCCAAGATAGT257730.7322442905373059No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC231030.6563861344928171No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC202360.5749309534517877No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC199040.5654984037114242No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC187820.5336209313960998No Hit
CCCACTGTGTTACTGCCATCGACTTACATTGGTGGTCTTTTTGTAATAGT167930.4771108668371155No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC156970.44597208817615686No Hit
AGGAAACACAATTAGCCTGGACAATAAATGAACACTTACAGTAGCAGTTC146660.41668004365111266No Hit
CCTGAGAGTGGAGCTGGAGAGACTGAGAGGGGCTCAGAGCCCTAGCGCCT146050.41494695469279297No Hit
GGTGCCCTACTACCTGGAGAACGAGCCCAGCGGCTACACGGTGCGCGAGG135950.3862515473501212No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT115060.32690035335126844No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC103900.29519334880233605No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC101450.2882325816746583No Hit
TGTTCTGGCGGTGTTTTGAAATTAGTTATTTCTCTTGCTTCTTTCTTGAT95800.2721802003394013No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAA93640.2660433607492854No Hit
CTCTCCACGCTCCCTCCACTCCCTCTGCAGGGCCCCTGTACGTGATCGTG85180.24200740568799797No Hit
GCTTCTTGGTCGTGTTCTTCATTCGGCACAGGATGACTGTTACCACCATA84840.24104142167844267No Hit
TCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCCTCTCCCTCCCTCC81730.23220550912045165No Hit
GCATCAAAGCAGAGGACAAGATCCACAAGCTGGCTGGGTCATGGGGGAGG79730.2265232502407147No Hit
CCTTCTCCGCACATTTTACAAGATGGCTTGTCCCAGTGGTGGATGTAGAT74870.21271536116295384No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACCGTCAAACTT71520.2031975775393944No Hit
GTGGCGGAGGGACTGGGGACCCGGGCACCCGTCCTGCCCCTTCACCTTCC67290.19117960000875067No Hit
TCACATCGTCCCCCTTTTTAGGTAGATGAAGGATAGTCACATATTTGTTA65420.1858666879561966No Hit
GCCCAGGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGC64410.18299714722192945No Hit
CCCGAGACCCCCAGCGCTACCTTGTCATTCAGGTACAAATTGCAGTCTGT62330.17708759798700296No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGATGGTGAGTCTT60900.17302478288799103No Hit
GACTAGTTTGGAACCTGCAGCCGATTCCTGGCTTTTCATCTCTTTCAATT60090.17072346804169755No Hit
AATTGCCCCGCAGCCACCCACCTCCCGCCCGCAGGTTGGAGGCAGGCGCC59790.16987112920973702No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGAACAATAAGGCTG57770.16413204774120266No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCACTTTTAGCTGA56390.16021128911418414No Hit
CTTTCCATTCTCTGCTGGATGACGTGAGTAAACCTGAATCTTTGGAGTAC55410.15742698226311302No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTCACAGATAACTCC54980.15620529660396956No Hit
CCCATCCAGTTCTGCCCCATCTCCCTCACGTTGCTGGTTTTCTTATAGTA53820.15290958645372213No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTCACAGTGAGCGTA53200.15114808620100367No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACAGAGAGTTTA52290.14856265841072333No Hit
CCGCGGAAAGGAAGGGGAGGGATGGAGAGGCCAGCTGCAGCTATGGAGTC50930.1446987223725022No Hit
GAGGGGTGCAGCATTGGTGAACATTCTTTTGAACATGAACCTGAAAACTC50920.14467031107810352No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAT49360.14023814915190866No Hit
GAAGGATAGGACAGGGTGGGCTGGGCCAGGCTGCATGCGCAGAGGGACAG49270.13998244750232047No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACTGTCAGTCTG45410.12901568786442813No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGCACTTGGAGTCTT45300.1287031636260426No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTTACTTGTAAAGTT45150.12827699421006233No Hit
ACTCACACTCACAAGCCTCACAGCCAGGCCTTCATTTATACCCCAAACAC44710.12702689725652017No Hit
TGCTAAGTGGCTAGGGACGTAGGGCTATATAATACCAGAAAACTCAATGA44590.12668596172373597No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAA44470.12634502619095175No Hit
GAGGGCCCACAGAGGAGGACTCACGGGGACACTTCTTCACGCAGGTGGCA42130.11969678330165946No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTAACAAATAGTCTT40800.11591808114663439No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGACCGAGAGTTTG40620.11540667784745806No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC40520.11512256490347121No Hit
CCCGAGACCCCCAGCGCTACCTTGTCATTCAGGGGGATGAAAGAATGCAT39820.11313377429556326No Hit
CACAGAGCCCAGACCTCCCAGACCCACTTAATGCTTGACTACAATCCAGC39800.11307695170676589No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA38600.1096675963789237No Hit
ACCATGTAGGGTCTTGAATGAGGAGTTACTTCATTTCCTCCAATAATTTT38390.10907095919655133No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGATGATTAGATGA37790.10736628153263023No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTTACTGTCAGTTTC37200.10569001516310783No Hit
GAGTTGTTGAGGAAGCCAGACTGCAGGTACTTGGGGGGCGCCAGGTAGCC36960.10500814409753938No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTCACCACCAGCTGA36770.10446832950396438No Hit
GAACCAAGTTCTTCCGAGGGAATTCCCACTTTGGATCCTCCTATGTTGGA35860.10188290171368405No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC35210.10003616757776952No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)