FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820488
Sequences flagged as poor quality0
Sequence length36-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTTTATTATATACATATCC516921.8327324916822905No Hit
ACGCGTGATCAGCAAAAGCAGGAAATAGAATCGGTAAAGCTAGAAAATCT367881.3043132961388242No Hit
ACGCGTGATCAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAA124780.44240571135207807No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT120740.42808194893933244No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACAC80760.2863334288250828No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGTTGA76350.2706978366864174No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA73430.2603450183088884No Hit
CGCGTGATCAGTAGAAACAAGGGTGTTTTTCTTTATTATATACATATCCT44880.15912140026832236No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACC40580.14387581156168722No Hit
CGCGTGATCAGCAAAAGCAGGAAATAGAATCGGTAAAGCTAGAAAATCTT30990.10987460326014505No Hit
ACGCGTGATCAGTAGAAACAAGGGGCCCTTGGGACTGGGATGTGGGGTAG29730.10540729122052636No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)