FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820488
Sequences flagged as poor quality0
Sequence length36-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTTTATTATATACATATCC471021.6699946959533245No Hit
ACGCGTGATCAGCAAAAGCAGGAAATAGAATCGGTAAAGCTAGAAAATCT413031.4643919775584935No Hit
ACGCGTGATCAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAA128850.45683583833719554No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT106390.3772042284881198No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACAC78360.27782426303533286No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA76620.27165511783776425No Hit
CGCGTGATCAGTAGAAACAAGGGTGTTTTTCTTTATTATATACATATCCT57530.2039717949517956No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGTTGA57350.20333360751756432No Hit
CGCGTGATCAGCAAAAGCAGGAAATAGAATCGGTAAAGCTAGAAAATCTT47730.1692260346436503No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACC40620.14401763099151638No Hit
ACGCGTGATCAGTAGAAACAAGGGGCCCTTGGGACTGGGATGTGGGGTAG32380.1148028284467085No Hit
ACGCGTGATCAGCAAAAGCAGGCAGGAAATAGAATCGGTAAAGCTAGAAA29890.10597456893984304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)