FastQCFastQC Report
Thu 28 Jul 2022
EGAF00006469250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983946
Sequences flagged as poor quality0
Sequence length36-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCG40760.20544914024877692TruSeq Adapter, Index 18 (97% over 37bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCG39190.1975356184089688TruSeq Adapter, Index 18 (97% over 37bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGGTT30950.1560022298994025TruSeq Adapter, Index 18 (97% over 37bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGTTT26800.13508432185150201TruSeq Adapter, Index 18 (97% over 37bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGGTT25990.13100154943733347TruSeq Adapter, Index 18 (97% over 37bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGTTT21560.10867231265367101TruSeq Adapter, Index 18 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)