FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843512
Sequences flagged as poor quality0
Sequence length36-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGCAAAAGCAGGACTGGTTGATATCCTGGGTGCCAGCGGT93470.32871322505408807No Hit
ACGCGTGATCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTT91620.3222071860431748No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTTTATTATATACATATCC67360.23689015555411758No Hit
ACGCGTGATCAGCAAAAGCAGGAAATAGAATCGGTAAAGCTAGAAAATCT42480.14939272280194352No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG35000.1230872245307915Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG32010.11257205877801817Illumina Single End PCR Primer 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)