FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783484
Sequences flagged as poor quality0
Sequence length36-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC32110.4098360655737705Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC30050.383543250404603Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTACGTATTCGCGCGGTTGATTTCCAGCATGATTTCCGCCGATAGGATG13900.17741268487933387No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG9420.12023219363764927TruSeq Adapter, Index 2 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC8140.1038949104257394Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)