FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006469259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5380180
Sequences flagged as poor quality0
Sequence length36-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAG4574268.502057551977815No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG2814485.231200443107851No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG1811013.366076971402444No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC1539242.8609451728380835No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC1438932.6745015966008574No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC635751.1816519149916918No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC546281.0153563635417402No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC293760.546004037039653No Hit
GAGTATGCCTGCCGTGTGAACCATGTGACTTTGTCACAGCCCAAGATAGT281050.5223802921091859No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC247440.4599102632253939No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC216080.40162225055667283No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC208560.38764502302896925No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC203670.3785561077882153No Hit
CCCACTGTGTTACTGCCATCGACTTACATTGGTGGTCTTTTTGTAATAGT191920.35671668977617854No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC166350.30919039883423977No Hit
CCTGAGAGTGGAGCTGGAGAGACTGAGAGGGGCTCAGAGCCCTAGCGCCT165710.3080008475552863No Hit
AGGAAACACAATTAGCCTGGACAATAAATGAACACTTACAGTAGCAGTTC159880.2971647788735693No Hit
GGTGCCCTACTACCTGGAGAACGAGCCCAGCGGCTACACGGTGCGCGAGG149450.2777788103743741No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT125100.23252010155794042No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC112300.20872907597887058No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC109900.204268258682795No Hit
TGTTCTGGCGGTGTTTTGAAATTAGTTATTTCTCTTGCTTCTTTCTTGAT107510.19982602812545305No Hit
CTCTCCACGCTCCCTCCACTCCCTCTGCAGGGCCCCTGTACGTGATCGTG102300.19014233724522228No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATCTGTGGGACAAGAGGATCAA101170.18804203576832001No Hit
GCTTCTTGGTCGTGTTCTTCATTCGGCACAGGATGACTGTTACCACCATA95900.17824682445568735No Hit
TCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCCTCTCCCTCCCTCC91730.170496154403756No Hit
GCATCAAAGCAGAGGACAAGATCCACAAGCTGGCTGGGTCATGGGGGAGG89980.16724347512536755No Hit
CCTTCTCCGCACATTTTACAAGATGGCTTGTCCCAGTGGTGGATGTAGAT85060.1580987996684126No Hit
GTGGCGGAGGGACTGGGGACCCGGGCACCCGTCCTGCCCCTTCACCTTCC77120.14334092911389582No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACCGTCAAACTT72860.13542297841336162No Hit
GCCCAGGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGC72000.13382451888226787No Hit
AATTGCCCCGCAGCCACCCACCTCCCGCCCGCAGGTTGGAGGCAGGCGCC69240.12869457899178094No Hit
CCCGAGACCCCCAGCGCTACCTTGTCATTCAGGTACAAATTGCAGTCTGT68770.12782100227129947No Hit
TCACATCGTCCCCCTTTTTAGGTAGATGAAGGATAGTCACATATTTGTTA68130.12663145099234596No Hit
GACTAGTTTGGAACCTGCAGCCGATTCCTGGCTTTTCATCTCTTTCAATT65720.12215204695753673No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGATGGTGAGTCTT64680.12021902612923731No Hit
CTTTCCATTCTCTGCTGGATGACGTGAGTAAACCTGAATCTTTGGAGTAC62270.11573962209442806No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGAACAATAAGGCTG60250.1119851008702311No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCACTTTTAGCTGA58600.10891828897917914No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTCACAGATAACTCC58060.10791460508756212No Hit
CCCATCCAGTTCTGCCCCATCTCCCTCACGTTGCTGGTTTTCTTATAGTA57980.10776591117769294No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTCACAGTGAGCGTA55550.1032493336654164No Hit
CCGCGGAAAGGAAGGGGAGGGATGGAGAGGCCAGCTGCAGCTATGGAGTC55100.10241293042240222No Hit
GGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACAGAGAGTTTA55020.10226423651253304No Hit
GAGGGGTGCAGCATTGGTGAACATTCTTTTGAACATGAACCTGAAAACTC54410.10113044544978048No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)