FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012979
Sequences flagged as poor quality0
Sequence length36-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC206272.036271235632723Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC178071.757884418137Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT69160.6827387339717802TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG50460.49813470960404904Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC43370.4281431303116846Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGAGCTGGCGGGAGTTGAACCCGCGTCCGAAATTCCTACATCCTCGGT15570.15370506200029813No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT10430.10296363498157415Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)