FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012979
Sequences flagged as poor quality0
Sequence length36-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG98940.9767231107456325Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG90810.8964647835739932Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG36290.35825026974892865Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG24240.2392942005707917Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG22200.21915557973067556Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT20320.20059645856429403Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT18230.17996424407613584Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTG17270.17048724603372825Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTG16640.16426796606839825Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGTG15730.15528456167403273Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGTG12800.1263599738987679Illumina Single End PCR Primer 1 (96% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG11640.11490860126419206Illumina Single End PCR Primer 1 (100% over 35bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG10600.10464185338491716Illumina Single End PCR Primer 1 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)