FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5431462
Sequences flagged as poor quality0
Sequence length36-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC167140.3077256178907263Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC150810.27766004806808925Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCAC101440.1867637111333928No Hit
AACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTAC94480.17394948174174835No Hit
CAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGC73940.13613277603709645No Hit
CTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCC73660.13561726106157052No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA66960.12328172414720015No Hit
AATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCG64230.11825545313582236No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA62960.11591722449682976No Hit
CCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGA59280.10914188481848902No Hit
TGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGA55670.10249542388402975No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG55510.10220084389801494No Hit
GGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAA55160.10155645017860752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)