FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5431462
Sequences flagged as poor quality0
Sequence length36-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCAC94500.1739863042400002No Hit
CAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGC78950.14535681184918536No Hit
AACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTAC78600.14471241812977795No Hit
CTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCC70010.12889715513060757No Hit
CGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG67030.12341060289108162No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG66140.12177200171887423Illumina Single End PCR Primer 1 (100% over 32bp)
TGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGA63360.11665367446186681No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG62240.1145916145597631Illumina Single End PCR Primer 1 (100% over 32bp)
CCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGA62010.11416815582986681No Hit
AATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCG59900.11028338226429643No Hit
GGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAA58780.10822132236219273No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA55020.10129869269084456No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)