FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590815
Sequences flagged as poor quality0
Sequence length36-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG93450.5874347425690606Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG91770.5768741179835493Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG30240.19009124253920162Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT29600.18606814745900685Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT24430.1535690825143087Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG17200.10812068028023371Illumina Single End PCR Primer 1 (100% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG16980.10673774134641677Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG16750.10529194155197179Illumina Single End PCR Primer 1 (100% over 35bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTG16260.1022117593811977Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)