FastQCFastQC Report
Wed 20 Jul 2022
EGAF00006469274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206998
Sequences flagged as poor quality0
Sequence length36-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC198111.6413448903809285Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC177471.4703421215279562Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC67600.5600672080649678TruSeq Adapter, Index 5 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC27710.2295778451994121Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG24540.20331433854902822Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)