FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206998
Sequences flagged as poor quality0
Sequence length36-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG101630.8420063662077318Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG84940.7037294179443545Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG35160.29130122833674954Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT24280.20116023390262452Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT20430.16926291510010788Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTG15370.1273407246739431Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTG15120.12526947020624724Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG13560.1123448423278249Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG12490.10347987320608651Illumina Single End PCR Primer 1 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)