FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006469282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24023824
Sequences flagged as poor quality0
Sequence length36-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGCAAAAGCAGGCTTTCTCGTTAGTGGCAAGTATGACCCA3140981.3074438107771684No Hit
ACGCGTGATCAGTAGAAACAAGGGCATTTTTCACTCAATTGCGTTCATTG3127891.3019950529108104No Hit
ACGCGTGATCAGTAGAAACAAGGCACTTTTTCCAGTTTATTTGCTGACAT1462560.6087956688327387No Hit
ACGCGTGATCAGCAAAAGCAGGACTAGCAGAAAAGTTACACTGTTGGTTT784060.32636769233740637No Hit
ACGCGTGATCAGCAAAAGCAGGAATACCAAGAGAATCCACTTGGATACAT645540.2687082622649916No Hit
ACGCGTGATCAGTAGAAACAAGGGCCTTTTTTCATTTTAATCATTTGTGT546780.22759906998985674No Hit
CGCGTGATCAGCAAAAGCAGGCTTTCTCGTTAGTGGCAAGTATGACCCAG529000.22019808336924213No Hit
CGCGTGATCAGTAGAAACAAGGGCATTTTTCACTCAATTGCGTTCATTGA524160.21818341659512658No Hit
CTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCC480150.19986410156850967No Hit
CAGGCTTTCTCGTTAGTGGCAAGTATGACCCAGATCTTGGAGATTTCAAA457700.190519211262953No Hit
CTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGTCATTGGACAAAGCA447300.18619017521939887No Hit
CTATACCTTTTCCTTTTTACTACTTTAACTGGTTTTCCTTGATAAAGCAA438550.18254795739429328No Hit
CACTATACCTTTTCCTTTTTACTACTTTAACTGGTTTTCCTTGATAAAGC419370.17456421592166177No Hit
CTTTCTCGTTAGTGGCAAGTATGACCCAGATCTTGGAGATTTCAAAACTA411320.1712133755225646No Hit
CGTTCATTGAATTAATGGATAAATTTATATTAGCTCAAGGCCCACCCCAT401580.1671590667663899No Hit
CTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGTCATTGGACA380880.15854262002585434No Hit
CTCAATTGCGTTCATTGAATTAATGGATAAATTTATATTAGCTCAAGGCC380720.15847601947133813No Hit
AGGGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTATA370750.15432597241804635No Hit
CAAAAGCAGGCTTTCTCGTTAGTGGCAAGTATGACCCAGATCTTGGAGAT369100.15363915419959787No Hit
TGAAATGTCATTGGACAAAGCACTATACCTTTTCCTTTTTACTACTTTAA365510.15214480425764026No Hit
AAATGTCATTGGACAAAGCACTATACCTTTTCCTTTTTACTACTTTAACT361070.15029663886981523No Hit
AAACAAGGGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAAT359470.14963063332465307No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT359450.14962230825533854No Hit
CGTGATCAGTAGAAACAAGGGCATTTTTCACTCAATTGCGTTCATTGAAT347810.14477711791428374No Hit
TTCTTTGTCTCTTAATTCCTTGTGAAATGTCATTGGACAAAGCACTATAC345050.14362825834887902No Hit
CCCACCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAA344850.14354500765573375No Hit
TCATTCTTTGTCTCTTAATTCCTTGTGAAATGTCATTGGACAAAGCACTA343670.14305382856617666No Hit
CAAACATCTTGCTTTATCAAGGAAAACCAGTTAAAGTAGTAAAAAGGAAA338170.14076443450468168No Hit
CGTGATCAGCAAAAGCAGGCTTTCTCGTTAGTGGCAAGTATGACCCAGAT327190.1361939714510063No Hit
CCTTTTCCTTTTTACTACTTTAACTGGTTTTCCTTGATAAAGCAAGATGT326940.1360899080845747No Hit
CAAGGGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA323510.13466215869713333No Hit
CTTTAACTGGTTTTCCTTGATAAAGCAAGATGTTTGCCTTTTCCCCTGGT318800.13270160487356217No Hit
TGATCAGTAGAAACAAGGGCATTTTTCACTCAATTGCGTTCATTGAATTA311990.1298669187719657No Hit
CATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTATATTAG308130.12826018039426196No Hit
ATCAGCAAAAGCAGGCTTTCTCGTTAGTGGCAAGTATGACCCAGATCTTG296820.12355235369689688No Hit
TACCTTTTCCTTTTTACTACTTTAACTGGTTTTCCTTGATAAAGCAAGAT295790.12312361262719873No Hit
ACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGTCATTGGAC292060.12157098720003943No Hit
ACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGTCATTGGACAAAGC290300.12083838110036103No Hit
TCTCTTAATTCCTTGTGAAATGTCATTGGACAAAGCACTATACCTTTTCC281880.11733352691894514No Hit
ACGCGTGATCAGCAAAAGCAGGAGACAATACAAAATGGAATGAATGTTTA281120.11701717428499309No Hit
CTACTTTAACTGGTTTTCCTTGATAAAGCAAGATGTTTGCCTTTTCCCCT274460.11424492620325556No Hit
ATCAGTAGAAACAAGGGCATTTTTCACTCAATTGCGTTCATTGAATTAAT272740.11352897024220623No Hit
CTTTGTCCAATGACATTTCACAAGGAATTAAGAGACAAAGAATGACAGTT267750.11145186544823171No Hit
TGATCAGCAAAAGCAGGCTTTCTCGTTAGTGGCAAGTATGACCCAGATCT264220.10998249071421769No Hit
CAAGATGTTTGCCTTTTCCCCTGGTTTCAGCTTTTCAAGTTCTTCAATAG249870.10400925348104449No Hit
CTGAAACCAGGGGAAAAGGCAAACATCTTGCTTTATCAAGGAAAACCAGT248090.10326832231205157No Hit
AAGAACTTGAAAAGCTGAAACCAGGGGAAAAGGCAAACATCTTGCTTTAT246170.10246911565785696No Hit
CGCGTGATCAGTAGAAACAAGGCACTTTTTCCAGTTTATTTGCTGACATT243020.10115791724081893No Hit
CTGGTTTTCCTTGATAAAGCAAGATGTTTGCCTTTTCCCCTGGTTTCAGC242410.10090400262672587No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)