FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24023824
Sequences flagged as poor quality0
Sequence length36-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGCAAAAGCAGGCTTTCTCGTTAGTGGCAAGTATGACCCA3060651.2740061698753704No Hit
ACGCGTGATCAGTAGAAACAAGGGCATTTTTCACTCAATTGCGTTCATTG2734081.1380702755731145No Hit
ACGCGTGATCAGTAGAAACAAGGCACTTTTTCCAGTTTATTTGCTGACAT1285840.5352353563695771No Hit
ACGCGTGATCAGCAAAAGCAGGACTAGCAGAAAAGTTACACTGTTGGTTT741610.3086977327173226No Hit
CGCGTGATCAGCAAAAGCAGGCTTTCTCGTTAGTGGCAAGTATGACCCAG674820.2808961637414593No Hit
CGCGTGATCAGTAGAAACAAGGGCATTTTTCACTCAATTGCGTTCATTGA609960.25389796395444786No Hit
ACGCGTGATCAGCAAAAGCAGGAATACCAAGAGAATCCACTTGGATACAT583370.24282978430078409No Hit
ACGCGTGATCAGTAGAAACAAGGGCCTTTTTTCATTTTAATCATTTGTGT474220.1973957185167524No Hit
CAGGCTTTCTCGTTAGTGGCAAGTATGACCCAGATCTTGGAGATTTCAAA465930.1939449772858809No Hit
CTATACCTTTTCCTTTTTACTACTTTAACTGGTTTTCCTTGATAAAGCAA424220.17658304523043458No Hit
CTTTCTCGTTAGTGGCAAGTATGACCCAGATCTTGGAGATTTCAAAACTA411580.17132160142365346No Hit
CTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCC392990.1635834494958005No Hit
CACTATACCTTTTCCTTTTTACTACTTTAACTGGTTTTCCTTGATAAAGC391370.1629091188813238No Hit
CAAAAGCAGGCTTTCTCGTTAGTGGCAAGTATGACCCAGATCTTGGAGAT390380.1624970279502547No Hit
CTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGTCATTGGACAAAGCA372600.1550960413296401No Hit
CAAACATCTTGCTTTATCAAGGAAAACCAGTTAAAGTAGTAAAAAGGAAA368880.15354757843713807No Hit
CGTTCATTGAATTAATGGATAAATTTATATTAGCTCAAGGCCCACCCCAT367380.15292319823854852No Hit
TGAAATGTCATTGGACAAAGCACTATACCTTTTCCTTTTTACTACTTTAA343300.14289981478385788No Hit
AAACAAGGGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAAT342970.1427624511401682No Hit
AGGGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTATA337060.14030239315772544No Hit
CTCAATTGCGTTCATTGAATTAATGGATAAATTTATATTAGCTCAAGGCC330930.13775075941282286No Hit
CGTGATCAGCAAAAGCAGGCTTTCTCGTTAGTGGCAAGTATGACCCAGAT330140.13742191917489904No Hit
CTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGTCATTGGACA329100.13698901557054363No Hit
AAATGTCATTGGACAAAGCACTATACCTTTTCCTTTTTACTACTTTAACT325320.13541557747009803No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT307910.12816860463180216No Hit
TTCTTTGTCTCTTAATTCCTTGTGAAATGTCATTGGACAAAGCACTATAC307610.12804372859208424No Hit
CGTGATCAGTAGAAACAAGGGCATTTTTCACTCAATTGCGTTCATTGAAT302710.12600408661002513No Hit
CCCACCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAA302400.12587504803564994No Hit
TGATCAGTAGAAACAAGGGCATTTTTCACTCAATTGCGTTCATTGAATTA302160.12577514720387561No Hit
TCATTCTTTGTCTCTTAATTCCTTGTGAAATGTCATTGGACAAAGCACTA297860.12398525730125229No Hit
CATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTATATTAG295010.12279893492393218No Hit
CCTTTTCCTTTTTACTACTTTAACTGGTTTTCCTTGATAAAGCAAGATGT294530.12259913326038352No Hit
CAAGGGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA292940.12193729024987862No Hit
ATCAGCAAAAGCAGGCTTTCTCGTTAGTGGCAAGTATGACCCAGATCTTG288000.11988099812919042No Hit
CGCGTGATCAGTAGAAACAAGGCACTTTTTCCAGTTTATTTGCTGACATT286950.11944393199017775No Hit
TGATCAGCAAAAGCAGGCTTTCTCGTTAGTGGCAAGTATGACCCAGATCT285110.11867802561324126No Hit
TACCTTTTCCTTTTTACTACTTTAACTGGTTTTCCTTGATAAAGCAAGAT284180.11829090989011574No Hit
CTTTAACTGGTTTTCCTTGATAAAGCAAGATGTTTGCCTTTTCCCCTGGT278230.11581420176904392No Hit
AAGAACTTGAAAAGCTGAAACCAGGGGAAAAGGCAAACATCTTGCTTTAT277760.11561856264015254No Hit
ACGCGTGATCAGCAAAAGCAGGAGACAATACAAAATGGAATGAATGTTTA275090.11450716588666317No Hit
CTGAAACCAGGGGAAAAGGCAAACATCTTGCTTTATCAAGGAAAACCAGT275020.11447802814406231No Hit
CTTTGTCCAATGACATTTCACAAGGAATTAAGAGACAAAGAATGACAGTT274500.11426157634188462No Hit
AGATTTCAAAACTATTGAAGAACTTGAAAAGCTGAAACCAGGGGAAAAGG270220.11248001150857583No Hit
ACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGTCATTGGAC254320.10586158140352678No Hit
ACCCAGATCTTGGAGATTTCAAAACTATTGAAGAACTTGAAAAGCTGAAA251720.10477932239263824No Hit
ACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGTCATTGGACAAAGC247300.10293948207412776No Hit
CAAGTATGACCCAGATCTTGGAGATTTCAAAACTATTGAAGAACTTGAAA246970.10280211843043806No Hit
AAACTATTGAAGAACTTGAAAAGCTGAAACCAGGGGAAAAGGCAAACATC245110.10202788698418704No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)