FastQCFastQC Report
Wed 20 Jul 2022
EGAF00006469288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1556704
Sequences flagged as poor quality0
Sequence length36-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC68160.43784817152136823Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC67900.43617797603140995Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG22340.14350833556026066TruSeq Adapter, Index 2 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)