FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006469302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3575138
Sequences flagged as poor quality0
Sequence length36-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGCACTTTTTCCAGTTTATTTGCTGACAT73940.20681719139233226No Hit
ACGCGTGATCAGCAAAAGCAGGACTAGCAGAAAAGTTACACTGTTGGTTT63490.1775875504665834No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC54470.15235775514120015Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCGTGATCAGCAAAAGCAGGCTTTCTCGTTAGTGGCAAGTATGACCCA51800.14488951195730065No Hit
ACGCGTGATCAGTAGAAACAAGGGCATTTTTCACTCAATTGCGTTCATTG51200.14321125506204235No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC39150.10950626241560467Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)