FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006469322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034477
Sequences flagged as poor quality0
Sequence length36-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC434732.1368145228478865Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC380881.8721273329705868Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG262291.2892256830625266Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC168710.829254889585874Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC161850.7955361500768994TruSeq Adapter, Index 5 (97% over 35bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT109630.5388608472840932Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA76980.3783773421867143Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG41110.20206667364634745Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAT28690.14101904322339354TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)