FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3171209
Sequences flagged as poor quality0
Sequence length36-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC127800.4030008744299099Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC108060.3407533215250083Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG80350.25337339796903957Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC56740.17892229745816185Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA49010.1545467359609537TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT34930.11014726560122653Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)